William Lin
Nancy Yu
Procedures:
1. prepare 3 plates of nutrient agar (covered)
2. Label the 3 plates for 3 different tests
3. Plate of Unknown:
a. Label the plate into 4 areas A, B, C, D
b. Prepare tape for further use
c. Fold the tape into a circle, use the clean side to quickly touch the target
d. Open the plate cover and quickly touch area A
e. Repeat step c and d for two more times with new tape and with different targets and full fill area B and C. Use a new clean tape to touch area D as the control
f. Seal the plate
4. Plate of Aseptic
a. Sterilize the sampling rod with burner till the sampling rod is red
b. Let it cool for ten seconds
c. Sterilize the mouth of test tube for a few seconds
d. Use the sampling rod to get some sample of aseptic in the test tube
e. Open the plate and spread the aseptic on top of the jelly with the sampling rod
f. Close the top and seal the plate
g. Sterilize the sampling rod again and put it away
5. Second plate of Aseptic
a. Repeat the procedure of 4
b. Label the plate into 4 areas E, F, G, H
c. Put the soaped ○ in area E
d. Put the ○ with Lysol in area F
e. Put the ○ without anything in area G
f. Put the sanitized ○ in area H
g. Close the top and seal the plate
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