Bacteria Lab Observations

Bacteria Lab Observations
----Group Member: Ashley Zheng, Nancy Yu, William Lin

1st plate: Aseptic (plain)
2nd plate: Aseptic (controlled)
3rd plate: Unknown

First Observation
-Our first plate was contaminated. We found a huge round colony of mold inside it. We supposed maybe it was contaminated because the cap of the plate was not closed well enough.
-The experiment for the second plate had not started yet.
-The third plate did not have anything in it. The reason might be the bacteria had not started growing or no bacteria were captured.

Second Observation
-The contaminated plate grew a small white colony and two yellow colonies, one big and one small.
-The second plate was still empty; maybe the growth had not started.
-The third plate grew a huge yellow colony and 3 small white colonies in area B which is the area for switch. Also, there were a red colony and a clash of white colonies in area C which is the area for door knob.

                                                    3rd plate in the 2nd obsevation

Final Observation
-The contaminated plate grew a new red colony and a new yellow colony.

      contaminated plate

-There were 2 huge white colonies growing in the second plate in the area F. Nothing was growing around the soaped and Lysol paper scrap. Also, many orange colonies were growing in area H and G. Some bacteria were growing around the indisposed paper scrap.
-The third plate remained almost unchanged except the colonies were a little bigger.

2nd plate in the final observation

Since we discussed together for the observations, our group members may have similar observations.

                                            

Bacteria Lab Procedure

My groupmates: Ashley Zheng (me)
                         William Lin
                         Nancy Yu

Procedures:

1.      prepare 3 plates of nutrient agar (covered)

2.      Label the 3 plates for 3 different tests

3.      Plate of Unknown:

a.      Label the plate into 4 areas A, B, C, D

b.      Prepare tape for further use

c. Fold the tape into a circle, use the clean side to quickly touch the target

d.      Open the plate cover  and quickly touch area A

e.      Repeat step c and d for two more times with new tape and with different targets and full fill area B and C. Use a new clean tape to touch area D as the control

f.  Seal the plate

4.      Plate of Aseptic

a.      Sterilize the sampling rod with burner till the sampling rod is red

b.      Let it cool for ten seconds

c. Sterilize the mouth of test tube for a few seconds

d.      Use the sampling rod to get some sample of aseptic in the test tube

e.      Open the plate and spread the aseptic on top of the jelly with the sampling rod

f.  Close the top and seal the plate

g.      Sterilize the sampling rod again and put it away

5.      Second plate of Aseptic

a.      Repeat the procedure of 4

b.      Label the plate into 4 areas E, F, G, H

c. Put the soaped ○ in area E

d.      Put the ○ with Lysol in area F

e.      Put the ○ without anything in area G

f.  Put the sanitized ○ in area H

g.      Close the top and seal the plate